Separation and preparation of epimers physanicantriol and 14-epi-physanicantriol from the leaves of Artemisia argyi / 国际药学研究杂志

ABSTRACT

Objective To establish a method for the preparative separation and isolation of the epimeric labdenetriols,phy?sanicantriol(1)and 14-epi-physanicantriol(2),from the leaves of Artemisia argyi.Methods The CH2Cl2fraction of 95% EtOH ex?tract from the leaves of A.argyi was separated by Diaion HP-20,silica gel and Sephadex LH-20 column chromatography and purified by semi-preparative HPLC to obtain an epimeric mixture of 1 and 2.Then,the epimeric mixture was separated by silica gel H column chromatography with eluting by n-hexane-CH2Cl2-isopropyl alcohol(1:1:0.15,V/V/V)to obtain compounds 1 and 2.The structures of 1 and 2 were identified by comparing MS and NMR data with those reported in the literature.Results and Conclusion Two diterpe?noids were isolated for the first time from the family Compositae.This method is effective,convenient and rapid,and is suitable for the separation and preparation of the epimers 1 and 2.