Antiviral effect of a Qingwen Jiedu formula on the influenza A virus FM1 strain in mice / 国际药学研究杂志

ABSTRACT

Objective To investigate the antiviral effect of a Qingwen Jiedu formula(QJF)on the influenza A virus FM1 strain using the virus-infected mouse model.Method BALB/c mice were randomly divided into the normal control group(to adminis-ter the same volume of saline),model control group(to administer the same volume of saline),positive drug control group[to admin-ister the 10 mg/(kg·d)of oseltamivir phosphate],and the 5,10 and 20 g/(kg·d)QJF-test groups,respectively.The mice in the nor-mal control group were intranasally instilled with PBS 20 μl,while the others were infected with the equal volume of diluted influenza virus solutions via the same intranasal way.After two hours of the virus infection,the drugs or saline solutions were orally administered to the mice once a day for successive six days.The mortality,average of survival time and protection rate on the animal death were de-termined to evaluate the protective effect of QJF on the virus-infected mice.Furthermore,the lung index,lung tissue viral load and se-rum levels of the inflammatory factors,TNF-α,IFN-γ,IL-6,were also detected as the related indicators for the effect of QJF. Re-sults Compared with the model control group,the mortality of the mice was significantly decreased together with the significant im-provement of the average survival time and protection rate in the 5,10 and 20 g/(kg·d)QJF groups(P<0.01).Further in the10 and 20 g/(kg·d)QJF groups,the lung index and lung tissue viral load decreased significantly both at the third day and sixth day of the vi-rus infection(P<0.01).Meanwhile,the serum IL-6 and TNF-α levels were also significantly decreased(P<0.01),while the serum IFN-γ levels increased significantly in the 10 and 20 g/(kg·d)QJF groups(P<0.01)than in the model control group,especially with a more significant increase in the 10 g/(kg·d)QJF group.Conclusion QJF showed an antiviral effect on the influenza A virus FM1 strain in the in vivo test in mice,which was thus expected likely to be developed as a new anti-influenza drug in future.