Significance of miR-145 expression in peripheral blood in short-term efficacy evaluation of radiotherapy for esophageal carcinoma / 国际肿瘤学杂志

ABSTRACT

Objective To investigate the relationship between the expression level of microRNA-145 (miR-145) in peripheral blood and clinicopathological characteristics of esophageal cancer patients and evaluate its significance in short-term efficacy of radiotherapy.Methods A total of 52 patients with esophageal.cancer diagnosed in Affiliated Hospital of Qingdao University were collected,and all of the patients rec.eived radiotherapy alone.The relative expression level of miR-145 in peripheral blood was detected by using the method of reverse transcriptase polymerase chain reaction (RT-PCR) before radiotherapy.The correlations between the expression of miR-145 and pathological characteristics as well as short-term efficacy of radiotherapy were analyzed.Results In the 52 patients,the relative expression of miR-145 [M(QR)] in peripheral blood of clinical stage Ⅰ-Ⅱ was 3.14 (1.44),higher than that of stage Ⅲ-Ⅳ [1.71 (0.48)],and the difference was statistically significant (Z =-3.002,P =0.003).The expression level of miR-145 was not related with the age (Z =-0.403,P =0.687),gender (Z =-1.179,P =0.238) and history of drinking (Z =-0.389,P =0.697).The expression levels of miR-145 in complete response (CR),partial response (PR) and no change (NC) groups were 3.27 (1.64),1.83 (1.26) and 1.40 (2.51),respectively,and the difference was statistically significant (H =6.567,P =0.038).Conclusion The expression level of miR-145 in peripheral blood is significantly correlated with the clinical stage of esophageal cancer.Its expression level is positively correlated with the short-term efficacy of esophageal cancer radiotherapy.High expression of miR-145 shows a better short-term efficacy of radiotherapy.