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Effect of Wutou decoction on depression and anxiety induced by neuropathic pain mice / 国际中医中药杂志
International Journal of Traditional Chinese Medicine ; (6): 327-333, 2018.
Article in Chinese | WPRIM | ID: wpr-693603
ABSTRACT
Objective To observe the effects of Wutou decoction(WTD)on Anxiety and Depression induced by the L5 spinal nerve ligation (SNL) mice. Methods The ICR male mice were randomly divided into sham,SNL,high-dose,medium-dose and low-dose group of Wutou decoction,and Pregabalin group,with 24 in each group. Except the sham group, SNL models were prepared in the remaining groups of mice. After successful modeling, the high, medium, and low dosages were administered with 12.60, 6.30, 3.15 g/kg of Wutou decoction,and pregabalin group was administered with 0.25 g/kg of pregabalin,and the sham group and the model group was given an equal volume of normal saline. All the administration was once per day, continuously last for 21 days. On the 7/14/21thdays after the operation, the depressive and eanxiety behavior were evaluated by the tests of the sugar water consumption, the open field and forced swimming detection. In addition, the number of the positive cells expressing the extracellular signal-regulated kinase (p-ERK) in hippocampus were analyzed on Day 7/14/21 in each group by Immunohistochemistry and the expression of both p-ERK and postsynaptic density protein 95 (PSD95) in the hippocampus were analyzed on Day 21 by Western blot. Results At 7, 14, and 21thday after administration, compared with the model group, the paw withdrawal mechanical threshold of mice(0.76 ± 0.21 vs.0.05 ± 0.03),(0.93 ± 0.33 vs.0.05 ± 0.01),(1.12 ± 0.20 vs.0.04 ± 0.01)in the high dose group of Wutou decoction respectively increased(P<0.01),and sugar consumption(1.86 ± 0.44 g vs. 0.84± 0.23 g), (1.84 ± 0.10 g vs. 1.02 ± 0.18 g), (1.63 ± 0.31 g vs. 0.75 ± 0.23 g) significantly increased(P<0.01),and immobility time 4 min after swimming(128.90 ± 35.27 s vs.180.30 ± 21.81 s),(125.50 ± 23.07 s vs.195.30 ± 27.70 s),(169.50 ± 23.07 s vs.207.50 ± 7.46 s)significantly decreased(P<0.01),and the retention time in the central area of the open field(35.35 ± 7.61 s vs.13.90 ± 2.27 s),(26.26 ± 3.61 s vs.13.08 ± 1.98 s),(24.04 ± 6.57 s vs.10.62 ± 3.38 s)significantly increased(P<0.01).After 21 days,the number of p-ERK positive cells in the high-dose group of Wutou decoction(11.00 ± 4.89 vs.33.67 ± 7.35)was siginificantly lower than that of the model group,and the hippocampus p-ERK protein(0.15 ± 0.09 vs.0.54 ± 0.04)siginificantly decreased,and the expression of PSD95 protein(0.32 ± 0.04 vs.0.57 ± 0.09)siginificantly decreased(P<0.01). Conclusions The Wutou decoction achieved significant anti-depressant and anti-anxiety effects,and regulated p-ERK expression levels of hippocampus in the late stage of NP.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Journal of Traditional Chinese Medicine Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: International Journal of Traditional Chinese Medicine Year: 2018 Type: Article