Effect of Shenling-Baizhu powder on gastrointestinal hormone, digestive enzyme and inflammatory factors in diarrhea-predominant irritable bowel syndrome rats / 国际中医中药杂志

ABSTRACT

Objective To investigate the effect of Shenling-Baizhu powder on gastrointestinal hormone,digestive enzyme and inflammatory factors in D-IBS rats.Methods A total of 50 Wistar rats were randomly divided into the normal group,the model group,the Shenling-Baizhu powder large dose group,medium dose group and small dose group,10 rats in each group.D-IBS rat model was induced by high lactose diet and restraint stress except normal group.At the same time,the Shenling-Baizhu powder groups received Shenling-Baizhu powder (large dose group9 g/kg,medium dose group3 g/kg,small dose group1 g/kg),normal group and model group received equal volume of normal saline.Malate dehydrogenase (MDH),lactate dehydrogenase (LDH) and disaccharidase activities were detected from small intestine of rats in each group.Plasma vasoactive intestinal peptide (VIP) and substance P (SP) activities were measured by radioimmunoassay.TNF-α and IL-10 levels in serum were determined by ELISA.Results Compared with the model group,the model group,the ratio of serum TNF-α/IL-10 (0.31 ± 0.15,0.34 ± 0.21,0.41 ± 0.15 vs.0.74 ± 0.19) significantly decreased in large,medium and low dose groups (P＜0.05).The disaccharidase activities in the small intestinal mucosa (38.44 ± 11.30 U/mg,44.77 ± 16.46 U/mg,42.58 ± 13.02 U/mg vs.23.23 ± 6.85 U/mg) significantly increased in the large,medium and low dose groups (P＜0.05).LDH activities in the small intestinal mucosa (1 863.61 ± 1 019.28 U/mg,1 136.13 ± 631.89 U/mg vs.416.50 ± 223.94U/mg) significantly increased in large,medium dose groups (P＜0.05).Plasma SP level (22.88 ± 10.17 pg/mg vs.31.86 ± 7.81 pg/mg) significantly decreased in large dose group (P＜0.05).Conclusions Shenling-Baizhu powder may increase the activities of LDH,MDH and disaccharidase,inhibit the abnormal secretion of SP and regulate of Th1/Th2 immune imbalance to relieve diarrhea.