Effect of Kuijietong on Phosphorylation of IkappaBalpha/NF-kappaB p65 Signaling Pathway in Rats with Ulcerative Colitis Induced by Dextran Sulfate Sodium / 广州中医药大学学报

ABSTRACT

Objective On the basis of previous research, we intended to further explore whether Kuijietong chieving the inhibitory effect on inflammatory action in ulcerative colitis(UC) through inhibiting the phosphorylation of IkappaBalpha/NF-kappaB p65(p-IκBα/NF-κB p65). Methods UC rat model was established by free drinking of dextran sulfate sodium(DSS) water. SD rats were randomly divided into normal group, model group, high-dose Kuijietong group(at intragastric dosage of 15 g·kg-1·d-1), low-dose Kuijietong group(at intragastric dosage of 5 g·kg-1·d-1), SASP group (at intragastric dosage of 0.3 g·kg-1·d-1). After treatment for 2 weeks, the expression levels of p-IκBα/IκBα and p-NF-κB p65/NF-κB p65 in colon tissues were determined by Western blotting method. Results The expression levels of p-IκBα/IκBα and p-NF-κB p65/NF-κB p65 in high-dose Kuijietong group were decreased significantly as compared with those in the model group and western medicine group, the difference being statistically significant(P<0.001). Conclusion Kuijietong has protective effects against inflammation in UC through blocking the activation of NF-κB classic inflammatory pathway, and the effect of high dosage of Kuijietong is the strongest.