Dynamic Accumulation Analysis of Contents of Total Flavonoids and Total Polysaccharides from Ixeris debilis A. Gray and Scorzonera sinensis Lipsch Harvested at Different Time / 广州中医药大学学报

ABSTRACT

Objective To evaluate the dynamic accumulation of contents of total flavonoids and total polysaccharides from above-ground parts of Ixeris debilis A. Gray and Scorzonera sinensis Lipsch harvested at different time, thus to provide reference for proper harvest period. Methods The orthogonal design was used to optimize the conditions of ultrasound-assisted extraction. Spectrophotometric method was used to detect the contents of total flavonoids and phenol-sulfuric acid method was applied to determine the contents of total polysaccharides in above-ground parts of Ixeris debilis A. Gray and Scorzonera sinensis Lipsch harvested at different time. Results The optimal extraction conditions for total flavonoids in above-ground parts of Ixeris debilis A. Gray were as follows ultrasonic power being 120 W, liquid to solid ratio being 30 1, extraction for 45 min at 60 ℃, and using volume fraction of 80％ alcohol as the solution; the optimal extraction conditions for total flavonoids in above-ground parts of Scorzonera sinensis Lipsch were ultrasonic power being 120 W, liquid to solid ratio being 30 1, extraction for 60 min at 80 ℃, and using volume fraction of 70％ alcohol as the solution. The optimal extraction conditions for total polysaccharides in above-ground parts of Ixeris debilis A. Gray and Scorzonera sinensis Lipsch were as follows ultrasonic power being 120 W, liquid to solid ratio being 30 1, extraction for 150 min at 80 ℃. With the prolongation of the harvesting time, the total flavonoids and total polysaccharides contents increases first and then declined, and arrived to the peak in July and August. Conclusion The optimized extraction processes are stable, reliable and simple for the extraction of the total flavonoids and polysaccharides in Ixeris debilis A. Gray and Scorzonera sinensis Lipsch. The optimal time for harvesting Ixeris debilis A. Gray and Scorzonera sinensis Lipsch is from July to August.