Effects of concentrated growth factors on proliferation and osteogenic differentiation in Beagle adipose-derived stem cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1-6, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-693768
ABSTRACT
Objective:
To investigate the effect of concentrated growth factor (CGF) on proliferation and differentiation in Beagle adipose-derived stem cells (ADSCs).Methods:
ADSCs were isolated from adipose tissue of healthy Beagles and cultured.The multidirectional differentiation potential of ADSCs was identified.The ADSCs were assigned to a CGF group and a control group.The rate of proliferation was analyzed by CCK-8 assay.The osteogenic differentiation capability was detected by ALP staining after the osteoinduction.Bone formationrelated gene expression was detected by RT-PCR.Results:
CGF promoted the proliferation of ADSCs in vitro.ADSCs in the CGF group showed higher level of ALP activity than that in the control group (P<0.05).CGF stimulated the expression of the genes associated with osteogenesis,such as Col-I and Runx2.Conclusion:
CGF can promote the proliferation and osteogenic differentiation in ADSCs in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2018
Type:
Article
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