Chlorogenic acid inhibits the inflammatory response of microglia cells infected with HSV-1 / 基础医学与临床
Basic & Clinical Medicine
;
(12): 983-987, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-694021
ABSTRACT
Objective To investigate the anti-inflammatory effect of chlorogenic acid( CGA) on BV2 microglia in-fected by herpes simplex virus ( HSV)-1.Methods BV2 cell was stimulated by HSV-1 to create a cell model of vi-rus encephalitis, then CGA was processed with different concentrations. MTT method was used to measure cell via-bility. Real-time quantitative PCR and ELISA were used to detect the content and expression of tumor necrosis fac-tor alpha ( TNF-alpha) and interleukin ( IL)-6. Results After CGA treatment, the cell survival rate significantly improved, and after HSV-1 infected BV2 cells, CGA inhibited the significant increase in mRNA levels of TNF-al-pha and IL-6. In addition, CGA can reduce the release of TNF-alpha and IL-6 induced by HSV in the cells.The mRNAs of TNF-alpha and IL-6 were also significantly inhibited by CGA.Conclusions CGA inhibits inflammatory response in HSE.CGA can be used as an anti-inflammatory agent to treat HSE new strategies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Basic & Clinical Medicine
Year:
2018
Type:
Article
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