Chlorogenic acid inhibits the inflammatory response of microglia cells infected with HSV-1 / 基础医学与临床

ABSTRACT

Objective To investigate the anti-inflammatory effect of chlorogenic acid( CGA) on BV2 microglia in-fected by herpes simplex virus ( HSV)-1.Methods BV2 cell was stimulated by HSV-1 to create a cell model of vi-rus encephalitis, then CGA was processed with different concentrations. MTT method was used to measure cell via-bility. Real-time quantitative PCR and ELISA were used to detect the content and expression of tumor necrosis fac-tor alpha ( TNF-alpha) and interleukin ( IL)-6. Results After CGA treatment, the cell survival rate significantly improved, and after HSV-1 infected BV2 cells, CGA inhibited the significant increase in mRNA levels of TNF-al-pha and IL-6. In addition, CGA can reduce the release of TNF-alpha and IL-6 induced by HSV in the cells.The mRNAs of TNF-alpha and IL-6 were also significantly inhibited by CGA.Conclusions CGA inhibits inflammatory response in HSE.CGA can be used as an anti-inflammatory agent to treat HSE new strategies.