Biological characteristics and biofilm formation of Klebsiella pneumoniae TH6 strain / 军事医学

ABSTRACT

Objective To explore the biological characteristics and biofilm formation of a strain of Klebsiella pneumoniae isolated from the stool of a patient with clinical inflammatory bowel disease .Methods The TH6 strain was identified as Klebsiella pneumoniae by 16S RNA, MALDI-TOF and automatic biochemical instrument .The morphology, growth rate, biofilm formation, capsid virulence factors and other biological characteristics were analyzed .Results and Conclusion The TH6 strain was short rod-shaped with a capsule under the transmission electron microscope , and was of K57 type according to the capsule serotype test .K.pneumonia TH6 grew rapidly under aerobic and anaerobic conditions , almost at twice the rate of standard strain ATCC2146, and reached the logarithmic growth phase at 2 h.The strain had multi-drug resistance.Biofilm formation was observed under a confocal laser scanning microscope (CLSM) at 4, 8, 12, 24, 48, 72, 96 and 120 h and the biofilm was formed at 72 h.The ability of fast growth , biofilm formation and other characteristics of K.pneumonia TH6 showed that this strain has a strong ability to adapt to the new environment is difficult to cure after colonization in vivo.