Cloning,expression and biological activity of m6A binding protein YTHDF2 / 军事医学
Military Medical Sciences
;
(12): 34-37, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-694311
ABSTRACT
Objective To construct the recombinant plasmid of YTH domain family 2(YTHDF2)and express it in E.coli in order to obtain YTHDF2 fusion protein that was capable of binding m 6A-modified RNA.Methods The coding region of YTHDF2 gene was amplified by RT-PCR.The recombinant plasmid pET-28a-YTHDF2 was constructed and expressed in E.coli.The fusion protein was purified by Ni2+-NTA resin affinity chromatography, while the fusion protein activity was analyzed by Ni2+-NTA magnetic spheres.Results and Conclusion The recombinant YTHDF2 protein was expressed in E.coli BL21(DE3)and purified.YTHDF2 fusion protein was capable of binding RNA with m 6A-modification. The preparation of YTHDF2 fusion protein provides an essential tool to study the biological function of RNA with m6A-modification.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Military Medical Sciences
Year:
2018
Type:
Article
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