Nitric Oxide-Mediated Cytotoxicity of Manganese in Basal Ganglia Neuronal Cells / 예방의학회지
Korean Journal of Preventive Medicine
;
: 459-466, 1999.
Article
in Korean
| WPRIM
| ID: wpr-69437
ABSTRACT
OBJECTIVES:
We have investigated to manifest whether manganese-induced neurotoxicity is mediated by nitric oxide(NO) in the rat primary neuronal cultures and assess the effect of Mn2+ on the N-methyl-D aspartate(NMDA) receptors.METHODS:
We have used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)assay to examine the effect of cytotoxicity of MnCl2 in neuronal cells . NO production was determined by measuring nirites, a stable oxidation product of NO. The neurons in the rat that contains neuronal nitric oxide synthase(nNOS) were examined by immunofluorescence and confocal microscopy. The effects of Mn2+ on the NMDA receptors was assesed by the whole cell voltage clamp technique.RESULTS:
We showed that the NO release and NOS expression were increased with 500uM MnCl2 treatment and an NOS inhibitors, NG-nitro-L-arginine , prevented neurotoxicity elicited by manganese. In the electrophysiological study, Mn2+ does not block or activate the NMDA receptors and not pass through the NMDA receptors in a neurons of basal ganglia.CONCLUSIONS:
It is concluded that manganese neurotoxicity in basal ganglia was partially mediated by nitric oxide in the cell culture model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Basal Ganglia
/
Fluorescent Antibody Technique
/
Receptors, N-Methyl-D-Aspartate
/
Microscopy, Confocal
/
Equidae
/
Cell Culture Techniques
/
Nitroarginine
/
Manganese
/
Neurons
/
Nitric Oxide
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Preventive Medicine
Year:
1999
Type:
Article
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