Effects of curcumin on acute kidney injury in endotoxic shock rabbits / 临床麻醉学杂志

ABSTRACT

Objective To investigate the effects of curcumin on acute kidney injury in endotoxic shock rabbits and discuss the possible mechanism.Methods Forty healthy male New Zealand white rabbits aged 2 months were randomly divided into 4 groupscontrol group (group C),curcumin control group (group Cur,curcumin 50 mg/kg),endotoxic shock group (group L,LPS 5 mg/ kg) and curcumin pretreatment group (group CurL,curcumin 50 mg/kg,LPS 5 mg/kg 30 minutes later),10 in each group.The concentrations of blood urea nitrogen (BUN) and creatinine (Cr) were detected,and the rabbits were sacrificed 6 h after LPS treatment.The pathological changes of renal tissue were examined and the pathological scores were recorded.SOD activities were measured by xanthine oxidase method.MDA contents were assayed according to thiobarbituric acid method.Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to investigate the mRNA levels of Nrf2 and HO 1.The expressions of Nrf2 total protein,Nrf2 nuclear protein and HO-1 protein in the renal tissue were determined by Western blot.Results No abnormal structures were visible in groups C and Cur.The histopathological changes of the kidneys included glomerular shrinkage,cellu lar swelling,vacuolization and desquamation in tubules,interstitial edema and massive inflammatory cells infiltration in group L.Compared with group C,the pathological manifestations was relieved obviously in group CurL.Compared with group C,the pathological score,the concentrations of BUN,Cr,and MDA were obviously increased,while SOD activity was significantly decreased,the mRNA expression of Nrf2 and HO-1,the protein levels of Nrf2 total,Nrf2 nuclear and HO-1 in the renal tissues were distinctly increased in groups L and CurL (P＜0.05).Compared with group L,the pathological score,the concentrations of BUN,Cr and MDA were obviously decreased,while SOD activity was significantly increased,the mRNA expression of Nrf2 and HO 1,the protein levels of Nrf2 total,Nrf2 nuclear and HO-1 in the renal tissues were significantly increased in group CurL (P ＜0.05).Conclusion Curcumin can ameliorate acute kidney injury induced by endotoxic shock in rabbits,and its mechanism may be relate to activation of Nrf2/HO-1 signaling pathway.