Study on the relationship between the expression of toll-like receptor 9-mRNA and inflammatory factors in patients with acute pancreatitis / 临床外科杂志

ABSTRACT

Objective To investigate the correlation between the expression of Toll-like receptor 9(TLR9)-mRNA and inflammatory factors in patients with acute pancreatitis(AP).Methods A total of 40 patients with AP diagnosed in our hospital from February 2016 to September 2016 in 24 h were enrolled.EDTAK2 anticoagulant venous blood was collected on day 1,3,5.And pancreatic elastase,proinflammatory cytokines and anti-inflammatory cytokines were detected.Peripheral blood monouclear cells were isolated and the mRNA expression of TLR9 was tested by polymerase chain reaction.The change of TLR9-mRNA in 5 days was analyzed.Results The level of TLR9-mRNA in peripheral blood mononuclear cells of patients with AP at 1,3,5 days were significantly higher than that in the control group,and the difference was statistically significant (P ＜ 0.05).Changes of the TLR9-mRNA expression in peripheral blood mononuclear cells in patients with AP was positively correlated with the levels of pancreatic elastase and proinflammatory cytokines,and the difference was statistically significant (P ＜ 0.05).Conclusion The expression of TLR9-mRNA in peripheral blood mononuclear macrophages was closely related to the up-regulation of inflammatory cytokines in AP patients,suggesting that TLR9 may mediate the occurrence and development of AP.To investigate the correlation between the expression of TLR9-mTLR9-mRNA and inflammatory factors in patients with AP.Methods A total of 40 patients with AP diagnosed in our hospital from February 2016 to September 2016 in 24h were selected.EDTAK2 anticoagulant venous blood was collected on day 1,3,5.And Pancreatic elastase,proinflammatory cytokines and anti-inflammatory cytokines were also detected.EDTAK2 anticoagulant venous blood was collected,tested the above indicators and used them as the benchmark level of the corresponding indicators.Results The levels of TLR9-mRNA in peripheral blood mononuclear cells of patients with AP at 1,3,5 days were significantly higher than in the control group,and the difference was statistically significant (P ＜ 0.05).Changes of TLR9-mRNA expression in peripheral blood mononuclear cells in patients with AP was positively correlated with pancreatic elastase and proinflammatory cytokine,and the difference was statistically significant (P ＜ 0.05).Conclusion The expression of TLR9-mRNA in peripheral blood mononuclear macrophages was closely related to the up regulation of inflammatory cytokines in AP patients,suggesting that TLR9 may mediate the occurrence and development of AP.