Expression of FFAR4 protein and mRNA in pancreatic cancer and its clinical significance / 临床与实验病理学杂志

ABSTRACT

Purpose To detect the expression of FFAR4 protein and mRNA in pancreatic cancer and to discuss its role and significance in the progression of pancreatic cancer. Meth-ods Immunohistochemistry was used to detect the expression of FFAR4 protein in paraffin-embedded tissues from 62 cases of pathologically confirmed pancreatic cancer. The relationship be-tween the expression of FFAR4 and clinicopathological factors of pancreatic cancer was also studied. At the same time, the ex- pression of FFAR4 in 20 pairs of pancreatic cancer tissues and adjacent tissues were detected using Western bolt and real-time quantitative PCR (qRT-PCR). Results The results of immu-nohistochemistry showed that the expression rate of FFAR4 pro-tein in pancreatic carcinoma was 75. 8% (47/62) significantly higher than that in paratumor tissue 40. 3% (25/62), and the difference was statistically significant (P＜0. 05). The high ex-pression of FFAR4 was related to the degree of pancreatic cancer differentiation, TNM stage, and lymph node metastasis ( P ＜0. 05). Western blot and qRT-PCR showed that the expression of FFAR4 protein and its mRNA expression in pancreatic cancer tissues was significantly higher than matched paracancerous tis-sues. There was a statistically significant difference between the two groups ( P ＜0. 001 ). Conclusion The dysregulated ex-pression of FFAR4 may be closely related to the progression of pancreatic cancer. It is hopeful that FFAR4 may become a new marker for the prognosis of pancreatic cancer after surgery and a new target for the study of clinical therapeutic drugs.