Comparison of ex vivo lung perfusion with cold storage in rat donor lung preservation / 上海交通大学学报（医学版）

ABSTRACT

Objective·To establish stable rat ex vivo lung perfusion (EVLP) model and compare the effect of preservation between EVLP group and cold storage group.Methods· Eight SD rats were reconditioned with EVLP for 4 h and 8 SD rats were under 3-h cold storage and 1-h EVLP.The biological index,wet-to-dry weight ratio and concentration of Evans blue were analyzed between two groups.Results · Wet-to-dry weight ratio of lung tissue in EVLP group was significantly lower (5.08±0.88 vs 6.09±0.48,P=0.012);Oxygenation index in EVLP group was significantly higher [(337.9±35.5) mmHg vs (300.5±21.6) mmHg,P=0.023];Concentration of Evans blue in EVLP group was significantly lower [(36.5±20.3) μg/mL vs (65.0±29.9) μg/mL,P=0.043].Conclusion · EVLP as an alternative to traditional cold storage technique can effectively alleviate the lung damage.