Antibacterial effect of Nd:YAG laser on Fusobacterium nucleatum / 上海交通大学学报（医学版）

ABSTRACT

Objective·To investigate the antibacterial effect of NdYAG laser on Fusobacterium nucleatum(F.nucleatum)in vitro. Methods·The laser effect on the biofilm formation ability of planktonic F.nucleatum were observed by crystal violet test;confocal laser scanning microscopy(CLSM) was used to detect the effect on the bacteria viability of mature biofilm; scanning electron microscopy (SEM) was performed to investigate the effect on the morphology of mature bioflm. Results·After 15 s radiation the biofilm formation ability of F.nucleatum went down.The viability of F.nucleatum in mature biofilm went down under 35 s iradiation. The deformation of mature biofilm and bacterium became distincted with the iradiation time raised. Conclusion·NdYAG laser shows the inhibition of biofilm formation ability on planktonic F.nucleatum.The vitality of F.nucleatum in mature biofilm is depressed after laser radiation. Laser can destroy the construction of the mature biofilm and bacteria.