Application of new markers in genetic analysis for hair stem without follicles / 复旦学报（医学版）

ABSTRACT

Objective To solve the problems of low success rate in detecting complete DNA typing of biological samples,such as naturally fallen hair,severely degraded bones and teeth which is difficult in forensic identification.Methods We applyed a specific retrotransposon (RE) DNA sequences in identification,with DNA fragment length of 60-125 bp.A RE combined with 21 loci were used in individual discrimination to compute the likelihood (LR) values and combined paternity index (CPI)values in phylogenetic identification.Results This research obtained high success rate in detection of hair stem without follicles and severely degraded biological samples.We found that most of the LR values were 108-1012,which met the requirement of identity establishing.Through the CPI values,it was difficult to support the assumptions,with most of the CPI values less than 10 000.Conclusions The RE with 21 loci can be used for individual discrimination,but more loci are need for applying in phylogenetic identification.