Effects of FHL1 on biological character of lung adenocarcinoma cell line A549 / 复旦学报（医学版）

ABSTRACT

Objective To explore the effect of expression of FHL1 on biological character of lung adenocarcinoma cell line A549.Methods Lung adenocarcinoma cell line A549 was transfected by FHL1 mRNA and shRNA lentivirus vector.The stable overexpression of FHL1 in lung adenocarcinoma cell line A549 model and low expression of cell line model were tested by Western blot and RT-PCR method.CCK-8 test,Transwell invasion,flow cytometry and Colony-forming Assay were used to test the proliferation,invasion,apoptosis and anchorage independence growth of the cell line.Results The difference between the stable overexpression of FHL1 group and its low expression group was obvious tested by Western blot and RT-PCR.CCK-8 test showed higher cell proliferation of the low expression group than that of the overexpression group and the blank group (P =0.002 2).Flow cytometry showed that the apoptosis rate of the overexpression group was 50.48 ％,significantly higher than that of the two low expression groups (16.41％,20.12％) and blank group (25.90％,P＜0.001).The invasiveness of the overexpression group was obviously lower than the low expression group and blank group in the Transwell invasion (P＜0.001).Colony-forming Assay showed that the colony number of the overexpression group was 28.7 ± 6.0,significantly less than the two low expression groups (157.0 ± 6.4,150.7 ± 9.5,P＜0.000 1).Conclusions The stable overexpression and low expression of FHL1 in lung cancer cell line A549 models were successfully constructed.The overexpression of FHL1 inhibited the proliferation,invasion and metastasis,and accelerated apoptosis in lung adenocarcinoma cell line A549.