Study on Effect of "Xuan-Jiang-Qing" Theory on Expression of T-bet and GATA3 among Young Rats Infected with RSV Based on Th Cell Balance / 世界科学技术-中医药现代化

ABSTRACT

This study was aimed to observe the method of "Xuan-Jiang-Qing" of Ding-Chuan (DC) decoction on the expression of T-bet and GATA3 of lung tissues,IFN-γ and IL-4 in serum of young rats infected with respiratory syncytial virus (RSV),in order to further explore the mechanism of Th1/Th2 immune balance.A total of 54 ealthy young Wistar rats were randomly divided into 6 groups,which were the normal group,RSV model group,DC decoction group,ascending (Xuan) therapy group,descending (Jiang) therapy group,pyretic clearing (Qing) therapy group,with 9 rats in each group.Except the normal group,rats in other groups were with RSV (Long bead) virus to establish the animal model.Two hours after intranasal medication,intragastric administration of corresponding medication was given to each intervention group.And equal volume of normal saline was given to the normal group and the RSV model group.Levels of T-bet and GATA3 protein in lung tissues were detected by Western blotting.Levels of INF-γand IL-4 cytokines in serum were detected by ELISA.The results showed that DC decoction and each decomposing agent group can adjust the general condition of RSV infection and reduce the lung index after RSV infection (P ＜ 0.05).Compared with the normal group,the expression of T-bet protein in lung tissues was decreased and the expression of GATA3 protein was significantly higher in the RSV model group and each intervention group with significant difference (P ＜ 0.05).The expression of T-bet protein was up-regulated and the expression of GATA3 protein was down-regulated in DC decoction and ascending (Xuan) therapy group compared to the RSV model group with significant difference (P ＜ 0.05).Compared with the normal group,the content of INF-γin serum of RSV model group was significantly decreased,and the content of IL-4 was increased with significant difference (P ＜ 0.05).Compared with the model group,DC decoction group can increase the content of INF-γ,and each intervention group can reduce the IL-4 content with statistical significance (P ＜0.05).It was concluded that the method of "Xuan-Jiang-Qing" of DC decoction can obviously increase T-bet and INF-γ cytokines,and inhibit GATA3 and IL-4 cytokines among RSV infected young rats.It can regulate Th1/Th2 cell immune balance.It is one of the important effectiveness mechanisms to prevent and treat RSV infection.