The Role of Mitogen-activated Protein Kinase in Enteritis Induced by Bacteroides fragilis Enterotoxin
Journal of Bacteriology and Virology
;
: 1-10, 2005.
Article
in Korean
| WPRIM
| ID: wpr-6962
ABSTRACT
A 20 kDa heat-labile toxin (BFT) produced by enterotoxigenic Bacteroides fragilis (B. fragilis) is associated with diarrhea and mucosal inflammation. Although intestinal epithelial cells are known to activate mitogen-activated protein kinase (MAPK) in response to bacterial infection, there has been little understanding on the association between MAPK activation and BFT-induced enteritis. This study was performed to investigate the role of MAPK in enteritis induced by BFT. In human colon epithelial cells, BFT increased IL-8 secretion in a dose-dependent manner. BFT activated the three main MAPK cascades, including extracellular signal-regulated kinase (ERK), p38, and c-Jun NH2-terminal kinase (JNK). BFT stimulation also activated AP-1 activation signals. Overexpression of dominant-negative plasmid of the c-Jun decreased the activated AP-1 signals and the up-regulated IL-8 expression induced by BFT stimulation. In addition, SB203580 and ERK inhibitor U0126 significantly reduced IL-8 secretion in colon epithelial cells stimulated with BFT. Furthermore, SB203580 significantly prevented BFT-induced severity of enteritis and fluid secretion in mouse ileum. These results suggest that MAPK activation may be required for IL-8 transcription in intestinal epithelial cells exposed to BFT and that the activated MAPK can mediate intestinal inflammation and mucosal damage induced by BFT.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphotransferases
/
Plasmids
/
Protein Kinases
/
Bacterial Infections
/
Bacteroides
/
Bacteroides fragilis
/
Interleukin-8
/
Colon
/
Transcription Factor AP-1
/
Diarrhea
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2005
Type:
Article
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