Research on protective function of aspirin-triggered lipoxins on acute kidney injury in mice / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate the renoprotective effect of aspirin-triggered lipoxins(ATL)on kidney of mice with acute kidney injury(AKI).Methods Eighty-eight male specific pathogen-free(SPF)C57BL/6J mice were randomly divided into lipopolysaccharide(LPS)groups(including 2 h group,4 h group,8 h group,12 h group, 24 h group),ATL+LPS(including 2 h group,4 h group,8 h group,12 h group,24 h group)and normal control group according to random numble table,and each group had 8 mice.The mice in LPS groups were given LPS intraperitoneal injection to establish AKI animal models,while the mice in ATL+LPS groups were given ATL intraperitoneal injection 30 minutes before LPS intraperitoneal injection.The enzyme linked immunosorbent assay was used to test the serum creatinine(Scr),serum urea nitrogen(BUN),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and urine neutrophil gelatinase-associated lipocalin(NGAL),kidney injury molecule-1(KIM-1),cysteine-rich protein-61 (Cyr61)and netrin-1 levels of mice.Results The kidney tissue injury scores of mice of ATL+LPS group[4 h(22.32 ± 1.04)scores,8 h(31.11 ± 1.86)scores,12 h(18.22 ± 0.92)scores,24 h(20.87 ± 3.18)scores] were lower than those of LPS group at the corresponding time points[4 h(35.47 ± 2.27)scores,8 h(52.28 ± 2.82) scores,12 h(54.99 ± 4.56)scores,24 h(53.41 ± 4.76)scores],and the differences were statistically significant(all P<0.01).The values of Scr,BUN,TNF-α and IL-1β in ATL+LPS group[Scr 8 h(143.07 ± 5.02)μmol/L, BUN 12 h(33.07 ± 3.52)mmol/L,TNF-α 4 h(196.33 ± 14.181)ng/L and 8 h(221.77 ± 10.11)ng/L,IL-1β 4 h(50.25 ± 2.67 ng/L)]were lower than those in LPS group at the corresponding time points[Scr 8 h(227.43 ± 11.17)μmol/L,BUN 12 h(59.68 ± 3.84)mmol/L,TNF-α 4 h(267.87 ± 26.48)ng/L and 8 h(334.78 ± 21.08)ng/L,IL-1β 4 h(89.45 ± 5.87)ng/L],and the differences were statistically significant(all P<0.01). The urine NGAL[4 h(56.76 ± 4.01)μg/L,8 h(65.44 ± 7.81)μg/L],KIM-1[8 h(78.19 ± 9.48)μg/L] and netrin-1[8 h(40.12 ± 2.01)ng/L,12 h(36.87 ± 2.87)ng/L]of mice in ATL+LPS group were lower than those in LPS group at the corresponding time points[NGAL 4 h(168.77 ± 10.77)μg/L,8 h(155.33 ± 8.26) μg/L;KIM-1 8 h(124.73 ± 13.47)μg/L;netrin-1 8 h(89.17 ± 2.74)ng/L,12 h(81.11 ± 3.88)ng/L],and the differences were statistically significant(all P<0.01).Conclusions ATL can treat LPS-induced AKI and play a renoprotective role in the kidney.