The effects of long non-coding RNA linc-01135 on the osteogenic differentiation capability of human inflammatory PDLSCs under static mechanical strain loading / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6): 193-197, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-697483
ABSTRACT
Objective:
To study the effects of long non-coding RNA linc-01135 on the osteogenic differentiation of inflammatory PDLSCs(P-PDLSCs) under 12% static mechanical strain loading.Methods:
Cells were isolated and cultured from the healthy and periodontitis samples respectively to obtain healthy PDLSCs(H-PDLSCs) and P-PDLSCs. RT-PCR were used to identify the expression level of linc-01135. Lentiviruses were used to upregulate and downregulate the expression of linc-01135, and the osteogenesis gene expression were analyzed by RT-PCR and the osteogenesis differentiation capability were evaluated by alizarin red staining.Results:
linc-01135 expression in P-PDLSCs was lower than that in H-PDLSCs. When the expression of linc-01135 was upregulated in P-PDLSCs before 12% SMS loading, the expression level of RUNX2, ALP, OPG was significantly increased. In contrast, the expression of RUNX2, ALP, OPG was significantly decreased when the expression of linc-01135 was suppressed. Alizarin red staining proved the same trend.Conclusion:
linc-01135 can promote the osteogenic differentiation capability of P-PDLSCs under 12%SMS loading.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2018
Type:
Article
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