Effect of Lipopolysaccharide and HMGB1 on murine macrophage polarization / 实用医学杂志

ABSTRACT

Objective This study was performed to investigate the infection of Lipopolysaccharide(LPS) and its downstream cytokine High-mobility Group Box 1(HMGB1)on the transcriptional regulation of macrophage polarization.Methods The murine macrophage cell line RAW246.7 was stimulated with LPS and HMGB1 respec-tively.The levels of M1 macrophage related mRNA(NOS2,TNF-α,IL-6)and M2 related mRNA(ARG-1,RELM-α)were determined by Q-PCR.Results The expression of M1 macrophage related mRNA(NOS2,IL-6,TNF-α) was significantly upregulated(P<0.01),while the M2 macrophage related mRNA(ARG-1,RELM-α)was down-regulated(P > 0.05)after the stimulation of LPS. Both the M1 and M2 related markers were upregulated at the mRNA level in HMGB1 stimulate group,and the upregulation of NOS2,TNF-α and RELM-α was statistically sig-nificant(P<0.01).Conclusions LPS induces the M1 polarization of murine macrophage,which involved in the inflammation reaction.HMGB1,as the downstream cytokine of LPS signaling,induces both M1/M2 polarization of murine macrophage. It′s inferred that HMGB1 intensifies periodontal inflammatory responses and,at the same time,involves the negative feedback mechanism in the responses to tissue remodeling.