Effects of RNAi-mediated CK2α gene silencing on epithelial-mesenchymal transition of laryngeal squa-mous cell / 实用医学杂志

ABSTRACT

Objective To investigate the effect of protein kinase CK2α expression on the transformation of epithelium in human laryngeal carcinoma Hep-2 cells.Methods HepG 2 cells were transfected into human laryn-geal squamous cell carcinoma cell line Hep-2 by immunization with pGCsi-H1-CK2α in vitro and divided into un-transfected group(pGCsi-H1-control)and interfering plasmid(PGCsi-H1-CK2α).The cells were transfected with G418 for 24 h and identified by Real-time PCR and Western blot.The invasion and migration ability of Hep-2 cells were detected by Transwell chamber.The patients were observed by phase contrast microscope the expression of E-cadherin,vimentin and transcription factors slug and snail were detected by Western blot. Results The expression of CK2α gene in the transfected group was significantly lower than that in the untransfected group and control plas-mid(P<0.01).Transwell experiments showed that stable knockout of CK2α prevented the migration and invasion of Hep-2 cells.Compared with untransfected and control plasmid transfection groups,the expression of E-cadherin in the interfering plasmid transfection group increased,but the expression of snail,slug and vimentin decreased. Conclusions RNAi mediated inhibition of CK2α inhibits the transformation of epithelial mesenchymal cells in la-ryngeal squamous cell carcinoma.