Role of Curcumin in Macraphage Polarization Induced by Breast Cancer Cells / 华中科技大学学报(医学版)

ABSTRACT

Objective To investigate the role of curcumin in macraphage polarization induced by breast cancer cells.Methods By using Transwell co-culture technique,murine breast cancer 4T1 cells were incubated with RAW264.7 mac-rophages for 5 days in vitro.Cells in the experiment group were pretreated with curcumin.Flow cytometry was used to detect the CD163 and F4/80 expression on macraphages and zymography to analyze the activity of MMP-9 secreted by co-cultured macrophages after treatment in the absence of serum for a night.Migration of macrophages towards breast cancer cell media was investigated by Transwell assay.Additionally,curcumin was orally administrated to mice bearing orthotopic transplantation tumors.Tumor growth was observed as well as overall survival.Furthermore,the CD163+/F4/80+macrophages in orthotopic transplantation tumor was measured by immunofluorescence.Results In vitro experiments showed that curcumin could dra-matically suppress the expression of CD163 and F4/80 on macrophages,the secretion of MMP-9 and the migration of macropha-ges.Furthermore,while the tumor growth was not significantly inhibited by curcumin,in vivo delivery of curcumin could pro-long the overall survival of tumor-bearing mice and downregulate the proportion of CD163+/F4/80+ macrophages in orthotopic transplantation tumors.Conclusion Curcumin could inhibit the macraphage polarization induced by breast cancer cells.