Effect of Fascin-1 on Invasion and Metastasis of Gastric Carcinoma / 华中科技大学学报(医学版)

ABSTRACT

Objective To investigate the expression of Fascin-1 in gastric carcinoma and its association with clinicopatho-logical features. Methods A total of 98 gastric cancer specimens were collected from our hospital from August 2014 to De-cember 2016.The expression of Fascin-1 mRNA and protein was detected in gastric cancer cell lines(MKN28,MKN45 and AGS)and gastric cancer tissues by real-time PCR,Western blotting and immunohistochemistry,respectively.The relationship between Fascin-1 protein expression and clinicopathological features was analyzed. Results Fascin-1 was highly expressed at mRNA and protein levels in MKN28 and MKN45 gastric cancer cell lines,and it was weakly expressed in AGS cell line.The ex-pression of Fascin-1 mRNA was much higher in gastric cancer tissues than in adjacent tissues.The expression of Fascin-1 was correlated with invasive depth,lymph node metastasis,lymphatic invasion,tumor differentiation and TNM stage(all P<0.05). Conclusion Fascin-1 is overexpressed in gastric cancer tissues.It is associated with the progression,invasion and malignancy of gastric cancer.It is expected to become a molecular marker for the progression of gastric cancer.