Effect of MiR-9 on Proliferation and Migration of Human Gastric Cancer Cells / 胃肠病学
Chinese Journal of Gastroenterology
;
(12): 342-346, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-698199
ABSTRACT
Background:
MicroRNA-9 (miR-9)is involved in the modulation of a variety of physiological process such as organ development and self-renewal and multipotential differentiation of cells. Moreover,its aberrant expression is closely associated with several types of malignant tumors.Aims:
To investigate the effect of miR-9 on biological behavior of human gastric cancer cells.Methods:
Expression of miR-9 in normal human gastric epithelial cell line GES-1 and human gastric cancer cell line BGC-823 and SGC-7901 was detected by real-time PCR. Then the two gastric cancer cell lines were transiently transfected with miR-9 mimic,miR-9 inhibitor and empty vector,respectively;the efficiency of transfection was assessed by real-time PCR. CCK-8 assay,Transwell assay and wound healing assay were used to examine the cell proliferation and migration capacities.Results:
The expression level of miR-9 was significantly higher in gastric cancer cells than in normal gastric epithelial cells (P<0. 05). Compared with cells transfected with empty vector,overexpression of miR-9 occurred in cells transfected with miR-9 mimic and effectively promoted the proliferation and migration of BGC-823 and SGC-7901 cells (P < 0. 05 ). Conversely,transfection with miR-9 inhibitor significantly suppressed the proliferation and migration of gastric cancer cells (P<0. 05).Conclusions:
Up-regulating miR-9 expression promotes the proliferation and migration of gastric cancer cells,which indicates that miR-9 overexpression may be associated with progression of gastric cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Gastroenterology
Year:
2018
Type:
Article
Similar
MEDLINE
...
LILACS
LIS