In vivo and in vitro hemostatic effects of composite coIIagen sponge / 中国组织工程研究

ABSTRACT

BACKGROUND: Collagen has good hemostatic effect, but the single collagen hemostatic material has no ideal hemostatic efficiency due to limited water absorption capacity and poor blood concentration effect. OBJECTIVE:To observe the hemostatic effect of composite collagen sponge in vivo and in vitro. METHODS: Collagen, sponge and calcium chloride were used as raw materials to prepare composite collagen sponge. (1) Cell toxicity testMouse L929 cells were cultured using composite collagen sponge extract, phenol solution and cell culture medium for 2, 4, 7 days. Then, cell proliferation was detected by MTT to evaluate cell toxicity. (2) The relationship between the material and the whole blood The relationship between the materials and the whole blood was observed by scanning electron microscope among composite collagen sponge, imported hemostatic materials and domestic hemostatic materials,following dripping the rabbit's anticoagulant blood onto the material surface.(3)In vitro coagulation activityBlood Clotting Index was used to detect the effect of coagulation among composite collagen sponge,imported hemostatic materials and domestic hemostatic materials, following dripping the rabbit's anticoagulant blood onto the material surface. (4) Liver leaves from 18 rabbits were drilled, and then randomly divided into three groups covered with composite collagen sponge, imported hemostatic materials and domestic hemostatic materials, respectively. Bleeding time and bleeding volume were measured. RESULTS AND CONCLUSION: (1) The cytotoxicity of composite collagen sponge was qualified as grade 1. (2) Compared with imported and domestic hemostatic materials, composite collagen sponge was conducive to blood water absorption but also to the further network hemoglobin and platelet clot formation, and the pores alternately extended to the interior to form the alternating complex sponge. (3) The blood clotting index of composite collagen sponge was significantly lower than that of imported and domestic hemostatic materials (P < 0.05). (4) The bleeding time and bleeding volume of composite collagen sponge were significantly lower than those of imported and domestic hemostatic materials (P < 0.05). Overall, the composite collagen sponge as a hemostatic material has good hemostatic effect and biocompatibility.