Polylactic acid/polyglycolic acid copolymer scaffolds carrying bone morphogenetic protein 2 gene enhanced adipose-derived stem cells promote cartilage defect repair / 中国组织工程研究

ABSTRACT

BACKGROUND:Gene-enhanced tissue engineering can promote the proliferation and differentiation of seed cells,reduce allogeneic immunity,promote vascularization,and facilitate the repair of osteochondral defects.OBJECTIVE:To observe the effect of lentivirus-mediated human bone morphogenetic protein-2 (BMP-2) transfected rebbit adipose-derived stem cells (ADSCs) cultured on polylactic acid/polyglycolic acid copolymer scaffold (PLGA) on osteochondral defect repair.METHODS:Thirty male New Zealand rabbits were randomly divided into control group (n=15) and experimental group (n=15).Animal models of bilateral femoral cartilage defects were made in all rabbits.The experimental group was implanted with BMP-2-enhanced ADSCs/PLGA copolymer scaffold,and the control group was implanted with ADSCs/PLGA copolymer scaffold.In both groups,autologous osteochondral mosaicplasty was then performed.After 3 months of implantation,bone tissues at defect region were taken for biomechanical and proteoglycans detection.Histological observation was done at 3,6,12 months after implantation.RESULTS AND CONCLUSION:(1) The compressive modulus and proteoglycan content of the experimental group were significantly higher than those of the control group at 3 months after implantation (P ＜ 0.01).(2) At 3,6 and 12 months after implantation,with the increase of postoperative time,the joint surface in the experimental group became more and more smooth,the color became more and more shallow,and the healing degree of the defect increased to different extent.However,there were no obvious changes in the joint surface,color,morphology and histomorphology in the control group.To conclude,BMP-2-enhanced ADSCs/PLGA copolymer scaffold could significantly promote the repair of osteochondral defects.