Correlation among oxidative stress and inflammatory factors ,apoptotic factors in rats with heart failure / 心血管康复医学杂志

ABSTRACT

Objective:To explore correlation among oxidative stress and inflammatory factors and apoptosis in rats with heart failure (HF).Methods:A total of 20 SD rats weighing 180～200g were randomly divided into sham operation group (n＝12) and HF model group (n＝8).After eight-week feeding ,cardiac function and hemodynamic indexes ,levels of su-peroxide dismutase (SOD) ,malondialdehyde (MDA) ,interleukin (IL)-10 and IL-18 ,inhibitory apoptosis protein Bcl-2 and pro-apoptosis protein Bax were measured and compared between two groups .Results:Compared with sham operation group ,there were significant reductions in levels of SOD [ (140.37 ± 7.84) KU/L vs.(80.89 ± 6.65) KU/L] ,IL-10 [ (63.04 ± 10.84) pg/ml vs.(25.24 ± 4.08) pg/ml] and Bcl-2/Bax [ (1.58 ± 0.20) vs.(0.45 ± 0.65)] ,and significant rise in levels of MDA [ (4.48 ± 1.26) μmol/mg vs.(15.99 ± 2.44) μmol/mg] and IL-18 [ (249.92 ± 24.65) pg/ml vs. (442.29 ± 32.98) pg/ml] in HF model group ,P＝0.001 all.Pearson linear regression analysis indicated that in HF rats , Bcl-2/Bax was significant positively correlated with SOD and IL-10 levels (r＝0.652 ,0.783 ,P＝0.001 both) ,and signif-icant inversely correlated with MDA and IL-18 levels (r＝ －0.644 ,－0.917 ,P＝0.001 both).Conclusion:During occur-rence and development of heart failure ,oxidative stress level rises and imbalance of inflammatory factor proportion occurs , which accelerate protein apoptosis ,then aggravate heart failure .