Clinical significance of the expression of pulmonary surfactant associated protein in bronchoalveolar lavage fluid of children with refractory Mycoplasma pneumoniae pneumonia / 新乡医学院学报

ABSTRACT

Objective To investigate the clinical significance of the expression of pulmonary surfactant associated pro-tein(SP)in bronchoalveolar lavage fluid(BALF)of children with refractory Mycoplasma pneumoniae pneumonia(RMPP). Methods Thirty children with RMPP were selected from January 2015 to December 2016 in the People's Hospital of Hebi City. The lung function of the children was detected in acute and recovery stage,and bronchoalveolar lavage was performed with fiexible bronchofiberscope. The BALF was collected,and the levels of SP-A,SP-B,SP-C and SP-D in BALF were detected by enzyme linked immunosorbent assay. Results The forced expiratory volume in one second (FEV 1 ),forced vital capacity (FVC)and FEV1 / FVC in RMPP children at acute stage were (1. 34 ± 0. 23)L,(1. 75 ± 0. 28)L and (68. 25 ± 6. 21)％respectively;and they were (1. 71 ± 0. 35)L,(1. 98 ± 0. 36)L and (88. 57 ± 8. 16)％ respectively in the children at recov-ery stage. The FEV1 ,FVC and FEV 1 / FVC in RMPP children at recovery stage were significantly higher than those in the chil-dren at acute stage (t = 4. 839,3. 070,14. 859;P < 0. 05). The levels of SP-A,SP-B,SP-C and SP-D in the RMPP children at acute stage were (50. 19 ± 10. 06),(42. 95 ± 12. 42),(36. 81 ± 8. 14)and (21. 57 ± 5. 46)μg·L - 1 respectively;and they were (135. 20 ± 18. 13),(108. 42 ± 20. 33),(142. 63 ± 21. 87)and (72. 69 ± 8. 54)μg·L - 1 respectively in the children at recovery stage. The levels of SP-A,SP-B,SP-C and SP-D in BALF of RMPP children at recovery stage were significantly higher than those in the children at acute stage (t = 22. 457,15. 052,24. 837,27. 623;P < 0. 05). Conclusion The detection of SP-A,SP-B,SP-C and SP-D levels in BALF plays a guiding role in the diagnosis,disease assessment,treatment and prognosis judgment of RMPP.