Roles of signaling lymphocytic activation molecule expression on CD4 +T cells surface in the pathogenesis of allergic conjunctivitis / 眼科新进展

ABSTRACT

Objective To discuss the function of signaling lymphocytic activation molecule (SLAM) expression on CD4 + T cells surface in the pathogenesis of allergic conjunctivitis.Methods Totally 10 patients with allergic conjunctivitis were selected as the observation group,and 10 individuals with no history of allergies receiving physical examination were selected as the control group,followed by the collection of venous blood.The expression of total IgE in the serum was tested by ELISA,and the expression of SLAM on CD4 + T cell surface was assessed by using flow cytometry.Then the correlation between SLAM expression on CD4 +T cell surface and serum total IgE level were analyzed.Next,20 Balb/c mice (2-month old) were equally divided into two groups (control group and model group) at random,with 10 mice in each group.Model group was firstly sensitized with the mixture of ragweed and complete Freund's adjuvant by footpad and root of tail injection on day 1.On day 7 and 14,intraperitoneal injection was enhanced,while rats in the control group were intraperitoneally injected with equal amounts of Freund's adjuvant.On day 15,mice were excited by ragweed pollen crude infusion drops,and,after 1 h,the eyes were taken for histopathology testing.Then,peripheral blood was collected,and the whole blood was used for the detection of SLAM expression,while the serum was used for the detection of specific IgE for ragweed pollen.Finally,the difference between the two groups and the correlation between SLAM expression and specific IgE level were analyzed.Results The expression of SLAM on CD4 +T cell surface in the observation group was significantly decreased when compared with the healthy controls (P =0.016),but the total IgE level was significantly increased in allergic conjunctivitis patients (P =0.002);and there was a negative correlation between SLAM expression and total IgE level (r =-0.453 4,P ＜ 0.05).About mice,the expression of SLAM on CD4 + T cell surface of the model group was significantly lower than that of the control group (P =0.000).The expression level of specific IgE of the model group was significantly higher than that of the control group (P =0.000).And there was a negative correlation between SLAM expression and specific IgE level in the model group (r =-0.527 4,P ＜ 0.05).Conclusion In allergic conjunctivitis,the expression of SLAM on CD4 + T cell surface in peripheral blood is significantly decreased,which is correlated with the production of IgE,and this may reflect the disease severity to some extent.