Efficacy Enhancing Effect of Lycopene on Human Steosarcoma Cells Treated by Cisplatin / 医学研究杂志

ABSTRACT

Objective To investigate the efficacy enhancing effect and mechanism of lycopene (LP) on human steosarcoma MG-63 cells treated by Cisplatin.Methods The human osteosarcoma MG-63 cells in logarithmic growth phase was treated with LP (l0μg/ ml),Cisplatin (40μg/ml) and LP (10μg/ml) + Cisplatin (20μg/ml),and blank group was set.The cellular growth inhibition rate was calculated by MTT.Cell cycle and apoptosis rate were analyzed by flow cytometry(FCM).The expression of Bax mRNA and bcl-2 mRNA were detected by RT-PCR and the ratio of Bax/bcl-2 was calculated.The expression of Caspase-3 protein was detected by western blotting.Results Compared with Cisplatin group,the cellular growth inhibition rate of human osteosarcoma M G-63 cell in LP + Cisplatin group was significantly increased (P ＜ 0.05).The cell cycle was arrested at G0/G1 phase(P ＜ 0.05).The apoptosis rate was significantly increased(P ＜ 0.01).The expression of Bax mRNA was increased,while the expression of bcl-2 mRNA was significantly decreased (P ＜ 0.05) and the ratio of Bax/bcl-2 was significantly increased (P ＜ 0.01).The expression of Caspase-3 protein was significantly increased(P ＜ 0.01).Gomparcd with blank group group,the G0/G1 phase in cell cycle of LP group was increased and the G2/M phase was decreased,and the expression of bcl-2 mRNA was significantly decreased (P ＜ 0.05).The ratio of Bax/bcl-2 was significantly increased (P＜0.05).The expression of Caspase-3 protein was significantly increased (P ＜0.01).Conclusion LP can effectively enhance the effect on human liver cancer MG-63 cells treated by Cisplatin,which perhaps was related to its effects of altering the cell cycle and the expression of apoptosis-related genes and proteins.