Prevention Effect of Curcumin Loaded Nano-liposomes on Diabetic Cardiomyopathy / 医药导报

ABSTRACT

Objective To investigate the prevention effect of curcumin loaded nano - liposomes on diabetic cardiomyopathy. Methods The curcumin-loaded nano-liposomes were prepared by Film dispersion and ultrasonic hydration technology and their quality inspections were also investigated.Sixty SD rats were randomly divided into normal control group,model control group,blank and curcumin-loaded nano-liposomes group ( n＝15). Diabetes model was induced by intraperitoneal single injection of STZ(70 mg·kg-1).After two weeks of STZ injection,the rats with model control were used for this study.The curcumin loaded nano-liposomes treatment group rats were treated with curcumin loaded nano-liposomes ( 5 mg·kg-1) via caudal vein administration for 12 weeks (three times a week).Rats of normal control group,blank nano-liposomes treated group and model control group were administrated equivalent volume of 0. 9% sodium chloride solution or blank nano-liposomes solution. After treatment for 12 weeks,the experimental animals underwent ultrasonic heart function examination.Then the rats were sacrificed and their hearts were arrested after saline perfusion. The myocardial cell collagen volume fraction ( CVF) and apoptosis index were detected. Results Curcumin loaded nano-liposomes showed good morphology and curcumin encapsulation efficiency ( 88. 37 ± 1.21) %with high stability and dispersibility. From the animal experiments, the evaluation indexes in curcumin loaded nano-liposomes treated group including LVIDd and LVFS were significantly higher than model control group and nano-liposomes treated group(P<0.05),and the LVPW,CVF and apoptosis index were significantly lower than model control group and nano-liposomes treated group(P<0.05). Conclusion Curcumin loaded nano-liposomes can improve the cardiac function of diabetic rats by reducing the fibrosis and apoptosis index of myocardial cells in diabetic rats, which could be used to prevent the diabetic cardiomyopathy.