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Promoting cell viability and migration of gastric cancer cells by PRRX2 via activation of Wnt/β-catenin signaling pathway / 中国病理生理杂志
Chinese Journal of Pathophysiology ; (12): 410-416, 2018.
Article in Chinese | WPRIM | ID: wpr-701136
ABSTRACT

AIM:

To study the effect of paired-related homeobox 2(PRRX2)gene on the viability and migra-tion ability of gastric cancer cells,and to analyze the underlying mechanism of regulating Wnt /β-catenin signaling pathway.

METHODS:

The expression of PRRX2 in gastric cancer and normal gastric tissue and the correlation between PRRX 2 ex-pression in gastric cancer tissues with the overall survival rate of gastric cancer patients were analyzed by bioinformatics. The small interfering RNA(siRNA)and over-expressed plasmids of PRRX2 were transfected into gastric cancer cells MGC-803 and SGC-7901,respectively.MTT assay and Transwell assay were used to detect the viability and migration ability of gastric cancer cells.Western blot and TOPflash/FOPflash dual-luciferase reporter gene assay were used to detect the activi-ty of Wnt/β-catenin signaling pathway.Co-immunoprecipitation was used to detected the interaction between PRRX 2 and β-catenin proteins.

RESULTS:

Knockdown of PRRX2 attenuated the viability and migration ability of gastric cancer cell line MGC-803(P <0.05).Over-expression of PRRX2 enhanced the viability and migration ability of SGC-7901 cells (P<0.05),increased the protein levels of β-catenin, c-Myc and cyclin D1(P<0.05)and the activity of TOPflash/FOPflash dual-luciferase reporter gene(P<0.05).PRRX2 interacted with β-catenin protein in gastric cancer cells.CON-CLUSIONPRRX2 promotes the viability and migration ability of gastric cancer cells, which may be related to Wnt/β-catenin signaling pathway.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Pathophysiology Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Pathophysiology Year: 2018 Type: Article