Effects of RARG on viability and migration ability of gastric cancer cells / 中国病理生理杂志

ABSTRACT

AIM:To investigate the effect of retinoic acid receptor gamma(RARG)on the viability and migra-tion ability of gastric cancer cells.METHODS:The expression of RARG in gastric cancer and normal gastric tissues and its correlation with the overall survival rate of gastric cancer patients were analyzed by bioinformatics.The expression of RARG was promoted and inhibited by over-expression plasmid transfection and RNA interference technique in gastric can-cer cells in vitro,respectively.MTT and Transwell assays were used to detect the effect of RARG on the viability and mi-gration ability of gastric cancer cells.The effect of RARG on regulating the Wnt/β-catenin signaling pathway was evaluated by Western blot and TOP/FOP dual-luciferase reporter assay.The protein interaction of RARG and β-catenin was deter-mined by co-immunoprecipitation and immunofluorescence co-localization assay.RESULTS:Over-expression of RARG en-hanced the viability and migration ability of gastric cancer SGC 7901 cells(P<0.05).Knockdown of RARG attenuated the viability and migration ability of gastric cancer MGC-803 cells(P<0.05).At the same time, RARG over-expression in-creased the protein expression levels of β-catenin, c-Myc, cyclin D1, Twist and Snail(P<0.05), and the activity of TOP/FOP dual-luciferase reporter gene(P<0.05).In addition, RARG interacted with β-catenin protein in the gastric cancer cells.CONCLUSION:RARG promotes the viability and migration ability of gastric cancer cells via activating the Wnt/β-catenin signaling pathway,thus playing an important role in the development of gastric cancer.