Up-regulation of miR-133a expression attenuates myocardial fibrosis in spontaneously hypertensive rats / 中国病理生理杂志

ABSTRACT

AIM:To investigate the effect of up-regulated expression of microRNA-133a (miR-133a) on myo-cardial fibrosis in spontaneously hypertensive rats (SHR). METHODS:Wistar-Kyoto (WKY) rats with homologous nor-mal blood pressure served as the normal control group. SHR were divided into SHR group, SHR+ adeno-associated virus (AAV) group and SHR+miR-133a-AAV group randomly. miR-133a carried by miR-133a-AAV was transfected into SHR heart by coronary perfusion. The rat tail artery pressure was monitored. The myocardial collagen deposition was observed by Masson staining. The expression of miR-133a in myocardial tissue was detected by real-time PCR. The protein levels of transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) were determined by immunohisto-chemistry and Western blot. RESULTS:Compared with the WKY rats, the tail artery pressure of the SHR increased sig-nificantly. The expression of miR-133a in heart decreased, and the expression levels of TGF-β1 and CTGF increased (P<0.05), and myocardial fibrosis occurred. After up-regulating the expression level of miR-133a in the heart of SHR, the myocardial fibrosis was significantly reduced, and the expression levels of TGF-β1 and CTGF decreased (P<0.05). CONCLUSION:Up-regulation of the miR-133a expression improves myocardial fibrosis induced by hypertension, which may be related to inhibiting the protein expression of TGF-β1 and CTGF in myocardium.