Screening of different metabolites in urine of children with Kaschin-Beck disease by the metabonomics technique / 中华地方病学杂志

ABSTRACT

Objective To search the different metabolites related with Kaschin-Beck disease (KBD) in urine of children with KBD by metabolomics technique,in order to provide clues in researching KBD biomarkers.Methods Among the children aged 7-15 years old in the KBD areas in Qinghai Province,the urine samples of 56 KBD children (case group),51 health children from the same area as the control group (internal control group) and 50 health children from non diseased areas (external control group) were examined by quadrupole-time of flight mass spectrometry (UPLC/QTOF-MS).Partial least squares discriminate analysis (PLS-DA) and multivariate statistical analysis were applied to explore and search the different endogenic metabolites associated with KBD.Results A obvious separation among three groups was observed,and the difference was significant among case group and internal,external control groups (P ＜ 0.05).Twenty-two different endogenic metabolites associated with KBD were found,compared with the external control group,21 metabolites were increased in the case group(P ＜ 0.05).Among them,14 kinds of metabolites (L-Cystine,isobutyryl carnitine,butyryl-L-carnitine,aspartyl-Asparagine,asparaginylAspartate and ubiquinone Q2,etc.) showed significant differences between the case group and internal control group (P ＜ 0.05).There were 4 kinds of metabolites showing significant differences between the two control groups(P ＜ 0.05).Condusion The metabolic profile in the urine of KBD children and healthy children is obviously different and the metabolites of L-Cystine,isobutyryl carnitine,butyryl-L-carnitine,aspartyl-Asparagine,asparaginyl-Aspartate and ubiquinone Q2 are more closely related with KBD,which can be considered as the clues in screening KBD biomarkers.