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Differential expression and clinical implications of circulating microRNA-133a and Galectin-3 in patients with chronic Keshan disease and dilated cardiomyopathy / 中华地方病学杂志
Chinese Journal of Endemiology ; (12): 362-365, 2018.
Article in Chinese | WPRIM | ID: wpr-701333
ABSTRACT
Objective To explore expression level of circulating microRNA (miR)-133a and Galectin-3 and their potential clinical application in differential diagnosis between patients with chronic Keshan disease and dilated cardiomyopathy.Methods Twenty-eight patients with chronic Keshan disease and 28 cases of age-and sex-matched healthy people as control from the same severe historical endemic areas of Keshan disease in Heilongjiang Province,and another 28 patients with dilated cardiomyopathy from non-affected areas were chosen for the study.All the subjects were asked for disease history and did physical examination,examined by Doppler echocardiography for left ventricular ejection fraction (LVEF) and left ventricular end-diastolic diameter (LVEDD),and collected fasting venous blood specimen (elbow vein).The plasma miR-133a and the serum Galectin-3 were determined by Real-time PCR and enzyme-linked immunosorbent method,respectively.Meanwhile,the correlation was analyzed between miR-133a,galectin-3,LVEF and LVEDD.Results The miR-133a and Galectin-3 levels in different groups were statistically different (F =48.789,9.485,P < 0.01).The plasma miR-133a level in chronic Keshan disease group and dilated cardiomyopathy group [median (quartile)0.394 (0.271,0.770),1.665 (0.943,2.713)] were both significantly lower than those in control group [2.382 (1.502,3.302],P < 0.01 or < 0.05],and the plasma miR-133a level in chronic Keshan disease group was lower than that in dilated cardiomyopathy group (P < 0.01).There was no significant difference of serum Galectin-3 level between chronic Keshan disease group and dilated cardiomyopathy group [17.710 (9.624,27.799),12.692 (9.376,26.290) μg/L,P > 0.05],but both were significantly higher than those in control group [8.070 (7.135,9.308) μg/L,P < 0.01].The miR-133a was positively correlated with LVEF (rs =0.297,P < 0.01),while negatively correlated with LVEDD,and Galectin-3 (rs =-0.271,-0.318,P < 0.05 or < 0.01);the serum Galectin-3 was negatively correlated with LVEF (rs =-0.392,P < 0.01),and positively correlated with LVEDD (rs =0.385,P < 0.01).Conclusion The combined application of miR-133a,Galectin-3,LVEF and LVEDD may provide assistance in clinical differential diagnosis of chronic Keshan disease and dilated cardiomyopathy.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Endemiology Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Endemiology Year: 2018 Type: Article