Partial purification and characterization of a novel murine factor that augments the expression of class I MHC antigens on tumor cells
Experimental & Molecular Medicine
;
: 93-99, 1998.
Article
in English
| WPRIM
| ID: wpr-70153
ABSTRACT
A soluble factor which augments the expression of major histocompatibility complex class I (MHC I) antigens on a number of murine tumor cell lines, has been isolated from the culture supernatants of mixed lymphocyte reaction of spleen cells derived from C57B1/6, Balb/c and Swiss mice. The factor, termed MHC-augmenting factor (MHC-AF) has been partially purified by Sephadex G-100 column chromatography and reverse phase HPLC. MHC-AF activity is associated with an 18 kDa molecule. MHC-AF activity was resistant to pH 2.0 treatment and partially purified MHC-AF preparations did not have any activity in L929 cell/vesicular stomatitis virus (VSV) interferon bioassay system. Antibodies to IFN-gamma did not block the activity of MHC-AF. These results indicate that a MHC-AF distinct from IFN-gamma, is produced by mouse spleen cells undergoing a mixed lymphocyte reaction.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spleen
/
Comparative Study
/
Tumor Cells, Cultured
/
Chymotrypsin
/
Lymphocytes
/
Trypsin
/
Histocompatibility Antigens Class I
/
Proteins
/
Interferon-gamma
/
Concanavalin A
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
1998
Type:
Article
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