Effects of Wnt/β-catenin signaling pathway on hypoxia induced proliferation and invasion of osteosarcoma cell in vitro / 局解手术学杂志

ABSTRACT

Objective To investigate the role of Wnt/β-catenin signaling pathway on hypoxia induced proliferation and invasion in os -teosarcoma SaOS-2 cell line and its mechanism.Methods Cultured SaOS-2 cells were treated with normoxia(21%O2)and hypoxia (1%O2)for 24 hours respectively.The proliferation rate were detected by CCK-8;the cell invasion ability were detected by Transwell inva-sion test;the mRNA and protein expression levels of Wnt/β-catenin were detected by RT-PCR and Western blot.Furthermore,β-catenin siR-NA was used to investigate the role of β-catenin on hypoxia induced proliferation and invasion.Results Hypoxia microenvironment signifi-cantly increased the proliferation of osteosarcoma SaOS-2 cell line and enhanced cell invasion potentiality.The mRNA and protein level of Wnt/β-catenin was significantly increased by hypoxia treatment.Targeted kept Wnt/β-catenin could obviously attenuated the proliferation and invasion ability of osteosarcoma SaOS-2 cell line induced by hypoxia.Conclusion Hypoxia promote the proliferation and invasion of osteosarco-ma SaOS-2 cell line by obviously activating Wnt/β-catenin signaling pathway.