Preparation of photoacoustic/ultrasonic real time dual-modality nanoparticles and imaging experiment / 中国介入影像与治疗学

ABSTRACT

Objective To synthesize a kind of carbon nanoparticles incorporated liquid-gas phase-transition nanodroplets (CNPs),and to verify their traceability for photoacoustic/ultrasound dual-modality imaging at real time.Methods The nanodroplets were synthesized using dual-emulsion technique.The basic properties were observed and measured with optical microscope,transmission electron microscope and laser particle size analyzer.The phase transition of CNPs was observed after laser irradiation.DiI-labeled CNPs phagocytosed by macrophages were also observed with the laser scanning confoeal microscope.The enhanced photoacoustic/ultrasound imaging with CNPs in vitro was observed after laser irradiation.And the in vivo experiment was used to analyze the effect of CNPs for tracking metastatic lymph nodes of the axilla in VX2 tumor-bearing rabbits with enhanced photoacoustic/ultrasound imaging.Results CNPs with the average diameter of (483.32 ± 45.09) nm and Zeta potential of (-26.3 ± 5.02) mV were successfully prepared.After laser irradiation,the phase-transition of CNPs obviously took place.After co-incubation with macrophages,CNPs were massively phagocytosed by macrophages.Photoacoustic and ultrasound imaging were significantly enhanced with CNPs in vitro and in vivo.And the mean photoacoustic signal and the grey level increased with the concentration of CNPs.Conclusion CNPs are successfully synthesized with excellent phase-transition feature after laser irradiation,which could be used for photoacoustic/ultrasound dual-modality imaging at real time.And this excellent property got further confirmed by tracking malignant metastatic lymph nodes in rabbits.