TIPE2 sensitizes CDDP to human non-small cell lung cancer cell line NCI-H1975 by suppressing AP-1 / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 40-43, 2018.
Article
in Zh
| WPRIM
| ID: wpr-702670
Responsible library:
WPRO
ABSTRACT
Objective:To investigate the chemosensitivity of TIPE2 in enhancing non-small cell lung cancer cell line NCl-H1975 and its mechanisms.Methods:TIPE2 lentiviral vector was transfected into NCI-H1975.IC50 was measured by CCK-8 method after treated with CDDP.Apoptotic cells were detected by Annexin V/FITC and PI apoptosis detection kit.The expression of AP-1 and MDR-1 were measured using Western blot after TIPE2 transfected.The mRNA expression of IL-1,IL-6 and TNF-α were measured using Real-time PCR after TIPE2 transfected combined with CDDP administration.Results:(1) TIPE2 reduces the values of IC50 of NCI-H1975 cells for CDDP(P<0.001).(2) TIPE2 increases the apoptosis rate of NCI-H1975 cells when treated with CDDP(P<0.05).(3) TIPE2 significantly reduces the expression of AP-1 and MDR1 in NCI-H1975 cells when treated with CDDP.(4)TIPE2 reduces the mRNA expression of IL-1,IL-6 and TNF-α in NCI-H1975 cells when treated with CDDP(P<0.01).Conclusion:TIPE2 may increase the chemosensitivity of non-small cell lung cancer cell line NCl-H1975 to CDDP by inhibiting AP-1 protein.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Immunology
Year:
2018
Type:
Article