Effects of miR-34 on migration and invasion of prostate cancer cells by MSR1 protein / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 544-548, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-702771
ABSTRACT
Objective:
To investigate the effect of miR-34a on the migration and invasion of prostatic cell line through MSR1.Methods:
Western blot was used to detect the expression of MSR1 in prostate cancer cell lines.qPCR was used to detect the ex-pression of miR-34a in prostate cancer cell lines.The correlation between miR-34a and MSR1 was examined by double luciferase as-say.Transwell invasion assay was used to detect the effect of miR-34a and MSR1 on the invasion ability of prostate cancer cells.The effects of miR-34a and MSR1 on the fine migration ability of prostate cancer were examined by scratch healing.Results:
The expression level of MSR1 in PC3 cells was relatively high by Western blot.The expression level of miR-34a in PC3 cells was relatively low by qPCR.Double luciferase assay showed a direct binding site between miR-34a and MSR1,MiR-34a could regulate the expression level of MSR1,Scaffold Healing Test and Transwell Invasion Test were used to detect the migration and invasion of PC3 cells after overexpressing miR-34a.Overexpression of MSR1 could reverse the inhibitory effect of miR-34a on the migration and invasion of PC3 cells.Conclusion:
miR-34a can regulate the migration and invasion of prostate cancer cells through MSR1 protein.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2018
Type:
Article
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