Salvianolic acid B induces autophagic cell death in giloma through mTOR/Ulk1 signaling pathway / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 549-552,563, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-702772
ABSTRACT
Objective:
To evaluate the effects and underlying molecular mechanism of salvianolic acid B(Sal B) on autophagy in glioma cell.Methods:
The C6 cells were treated with Sal B at a concentration ranging from 0 to 400 μmol/L and the cell viability were measured by CCK8 assay.Flow cytometry was performed for apoptosis,Western blot assay for protein expression and the expression of LC3 was calculated by immunofluorescence.Results:
The cell viability was weakened by Sal B at a concentration of 100 μmol/L, thus,the concentration of 10,20 and 50 μmol/L were selected for further experiment.Sal B dose-dependedly induced C6 cell apoptosis and the expression of caspase-3 and Bax,notably decreased the protein level of Bcl-2 and Ki67.Meanwhile,Sal B significantly up-regulated the expression level of Atg5,Atg7,Atg12 and Beclin1,increased the ratio of LC3Ⅱ/Ⅰand the amount of LC3-labeled puncta per cell,and down-regulated the expression of p62.In addition,the protein level of mTOR was makedly decreased by Sal B and Sal B induced the expression of Ulk1.Conclusion:
Sal B induces autophagic cell death in glioma cell through mTOR/Ulk1 signaling pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2018
Type:
Article
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