Effect of Gli2 on apoptosis and ROS level in renal tubular epithelial cells under high glucose / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effect of Gli2 on apoptosis and ROS level in renal tubular epithelial cells under high glucose. Methods:The renal tubular epithelial cell NRK-52E was used as the object of study,Gli2 overexpression vector and control vector were transfected,the non transfected cells were also used as controls,RT-PCR and Western blot detected Gli2 levels,culture of non transfected cells by high glucose and low glucose cell culture medium, cells transfected with Gli2 overexpression vector were cultured in high glucose medium,apoptosis was detected by flow cytometry,the ROS content was detected by DCFH-DA probe method, the content of SOD was detected by xanthine oxidase method; Bax, Cleaved Caspase-3 and Smo levels were detected by Western blot. Results:The Gli2 mRNA and protein levels of NRK-52E cells transfected with Gli2 overexpression vector were significantly higher than that of control cells(P＜0. 01),the Gli2 mRNA and protein levels in the NRK-52E cells transfected with the control vector were not different from those of the control cells(P＞0. 05). The apoptosis rate of NRK-52E cells without transfection was elevated after high glucose culture,elevated ROS content,SOD content decreased,the levels of Bax,Cleaved Caspase-3 were elevated in the cells,Smo levels declined,compared with the control cells,the difference was statistically significant(P＜0. 01). After overexpression of Gli2 over-expression vector,NRK-52E cells were cultured with high glucose,the rate of apoptosis decreased,ROS content decreased,elevated SOD content,the levels of Bax,Cleaved Caspase-3 decreased,elevated levels of Smo,compared with NRK-52E cells cultured with simple high glucose,the difference was statistically significant(P＜0. 01). Conclusion:Apoptosis and oxidative damage in renal tubular epithelial cells induced by high glucose, Gli2 can decrease the apoptosis of renal tubular epithelial cells induced by high glucose, mitigate oxidative damage.