Effects of different times bone marrow mesenchymal stem cell transplantation on treatment of brain injury in rats / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effects of exogenous BMSCs transplantation at different time points on the treatment of traumatic brain injury in rats. Methods: Male 3-week-old SD rats were taken,BMSCs was isolated and cultured in vitro to 3 genera-tions. Fifty healthy female SD rats were randomly divided into 5 groups,10 rats in each groupcontrol group,brain trauma model group, early treatment group,middle treatment group,late treatment group. A traumatic brain injury model was made by using cortical trauma instrument in rats,after the establishment,the treatment groupearly treatment group(1 day),middle treatment group(28 days),late treatment group model(56 days),were given BMSCs (1×106ml-1) intervention treatment,the control group and model group were given equal dose of normal saline. In addition,40 rats were tested by water maze,and each group was divided into four points6 d,7 d, 8 d and 9 d after injury,the spatial memory ability of rats was detected. All the experimental groups were sacrificed at 14 days after treatment. Morphological changes of brain tissue were observed by HE staining;the expression of LC-3 and Beclin-1 protein was detected by Western blot. Results: The brain trauma model group was compared with the control group,the shape of neuronal cell bodies appears to change,a clear gap was seen between the cells,and necrotic neurons appeared. Compared with the brain trauma model group,the morphological changes of neurons were alleviated,and the expression of protein was obviously weakened in the early stage of traumatic brain injury (1 d) and the medium term group (28 d) (P＜0. 05). BMSCs was not effective in the treatment of advanced traumatic brain injury (56 d). Conclusion: In the early and middle stage of traumatic brain injury in rats,the transplantation of BMSCs may alleviate the extent of traumatic brain injury to some extent,and the effect of late treatment is not obvious.