Regulation of STAT3 signaling pathway by PTEN on proliferation of cardiac fibroblasts / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effect of PTEN on proliferation of cardiac fibroblasts and its mechanism. Methods:Stimulation of cardiac fibroblasts by high glucose, the levels of PTEN in cells were detected by qRT-PCR and Western blot. Cell transfection of PTEN over expression vector,the levels of PTEN in transfected cells were detected by qRT-PCR and Western blot. High glucose stimulated transfection of PTEN overexpression vector into cardiac fibroblasts,cell proliferation was detected by MTT,the levels of p-STAT3 and STAT3 in cells were detected by Western blot,STAT3 pathway blocker AG490 was added into the cell culture medium to treat the cells, cell proliferation was detected by MTT, the levels of p-STAT3 and STAT3 in cells were detected by Western blot. Results:The levels of PTEN mRNA and protein in cardiac fibroblasts after high glucose treatment were significantly lower than those in normal culture ( P＜0. 05 ) . The expression of PTEN mRNA and protein in transfected PTEN overexpressing cells was significantly higher than that in non transfected cells( P＜0. 05) . The cell proliferation activity and p-STAT3 level were significantly higher than those of normal cells after high glucose(P＜0. 05). The expression of PTEN was increased after high glucose induction,the cell proliferation activity and p-STAT3 level were decreased, the proliferation of the cells treated with AG490 decreased further. Conclusion:PTEN slows down the proliferation of cardiac fibroblasts induced by high glucose by inhibiting STAT3 signaling pathway.