Differential expression profiling of A549 cells induced by Toxoplasma polymorphism effector ROP16 / 中国人兽共患病学报

ABSTRACT

The aim of this study is to explore the changes and significance of gene expression profile induced by ROPl6 of Toxoplasma gondii (T.gondii)type Ⅰ,Ⅱ,Ⅲ.The A549 cell line was used as the research model.And,stable transfected ROPl6 of type Ⅰ,Ⅱ,Ⅲ of T.gondii cell lines were established.Then,we used gene expression microarray to identify genes that were differentially expressed.Gene Ontology (GO)functional classification and pathway analysis were performed on screened differentially expressed genes.According to the results of the gene expression profile,ROP16Itransfer group owned 1 270 different genes,with 776 genes up-regulated and 494 genes down-regulated;ROP16Ⅲtransfer group owned 1 362 differ-ent genes,with 842 genes up-regulated and 520 genes down-regulated;ROP16Ⅱtransfer group owned 11 052 different genes, with 5 063 genes up-regulated and 5 989 genes down-regulated.The ROP16Ⅰ/Ⅲgroup has the similar expression spectrum. However,ROP16Ⅱtransfer group was very different,which has distinct molecular profiles.Pathway analysis showed 74 path-way changes of the ROP16Ⅱtransfer group,and 19 pathways was solely dominated by it.In addition,these pathways were re-lated to NF-kappa B,Toll-like receptor,Chemokine signa-ling pathway and inflammatory response,immune response and metabolic process.Above all,ROP1 6 could affect host cell gene expression profiles.And,compared to Toxoplas-ma gondii type Ⅰ,Ⅲ,type Ⅱ has unique gene expression profiles and signaling pathway.Understanding its unique signaling pathway is very important for the prevention of type Ⅱ T.gondii.